Text

UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BAWANG DAYAK (Eleutherine americana Merr.), BAWANG BOMBAY (Allium cepa linnaeus), BAWANG MERAH (Allium cepa var. ascalonicum), MENGGUNAKAN METODE DPPH (1,1-DIFENIL-2- PIKRILHIDRAZIL)

Share :
• 
• 
• 
• 
• 
• 
• 

ANTIOXIDANT ACTIVITY OF BAWANG DAYAK (Eleutherine americana Merr.), BAWANG BOMBAY (Allium cepa linnaeus), BAWANG MERAH (Allium cepa var. ascalonicum) EXTRACTS USING DPPH (1,1-DIPHENYL-2-PICRYLHYDRAZYL) METHOD Putri Sari Pertiwi Siahaan NIM: 1641006 ABSTRACT A research on the antioxidant activity had been done on bawang Dayak (Eleutherine americana Merr.), bawang Bombay (Allium cepa linnaeus), and bawang Merah (Allium cepa var. ascalonicum) using DPPH (1,1-diphenyl-2- picrylhydrazyl) method. Simplicia was extracted using methanol, dichloromethane and hexane. The macerate was then concentrated using vacuum rotary evaporator. The data shows that bawang Bombay extracted with methanol gives the highest yield (61.27%) and bawang Merah extracted with hexane gives the lowest (0.27%). On the antioxidant test, DPPH method was used where extracts were diluted using ethanol making them into 7 various concentrations which are 12,5, 25,0, 50,0, 100,0, 200,0, 400,0, and 800,0 ppm. antioxidant activity was measured using spectrophotometric at a wavelength of 517. The data obtained were analyzed using 3 x 3 x 7 factorial Analysis of Variance The results showed that there were significant differences in antioxidant activity based on the type of plant with a value p = 0,000, based on the variety of solvents with p = 0,000, and based on variations in concentration of the extracts with p = 0,000. It was shown also that there is a significant difference in the interaction between those three variables with p = 0,000. Duncan's multiple range test results showed that bawang Dayak has the highest antioxidant activity with mean percentage of 35,003%, followed by bawang Bombay (15,362%) and bawang Merah (10,904%). The result also showed that Methanol is the best solvent which give antioxidant activity with mean percentage of 25,576%, followed by dichloromethane (23,495%) dan hexane (12,198%). Therefore, this study concluded that bawang Dayak and Methanol provide the highest antioxidant activity. Key Words: Bawang Dayak, Bawang Bombay, Bawang Merah, Antioxidant Activity, DPPH (1,1-Diphenyl-2-Picrylhydrazyl)